|
KMID : 0614020000160010057
|
|
Journal of Pharmaceutical Sciences (C.N.U.)
2000 Volume.16 No. 1 p.57 ~ p.63
|
|
|
Identification of Diiodotyrosine-1375 in 50K Polypeptide of Bovine Thyroglobulin in Comparison with Synthetic Peptide
|
|
Liu-Xi-Wen
Sok Dai-Eun
|
|
|
Abstract
|
|
|
|
A peptide fragment containing residues 1218-1591, prepared from thermolysin-mediated proteolysis of bovine thyroglobulin, was reduced by dithiothreitol and then treated with iodoacetic acid. The carboxymethylated peptide was digested with endoproteinase Asp-N, and fractionated by RP-HPLC. The fractions were analyzed by LC/ESI-MS for the monitor of a peptide with a hormonogenic site at Tyr-1375, and a fraction was found to contain to a peptide (residues 1366-1381) containing Tyr-1375. This observation was positively confirmed by the comparison with synthetic peptide, DVEEALAGK (diiodotyrosine) LAGRFA, which was produced from the oxidative iodination of DVEEALAGKYLAGRFA by lactoperoxidase employing KI and (H_2)(O_2)-generating system.
|
|
|
KEYWORD
|
|
|
Thyroglobulin, diiodotyrosine, peptide, lactoperoxidase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|